If precipitation occurs, a gentle warming of the peptide solution would be helpful (Avoid excessive warming of the sample!).Sonication improves solubility and minimizes peptide aggregation. Chill the tube on ice between sonications. Briefly Sonicate the mixture to aid peptide dissolution (3x 10sec, ice between).Use sterile and ideally oxygen-free water or buffer (Tris or phosphate, buffer at pH 7) when preparing peptide solutions.Allow peptides to warm to room temperature before reconstituting.Centrifuge to spin down any powder on the tube sidewall before use (10000xg for 5 min).We recommend to dedicate 1 aliquot tube of 1 mg for this purpose).
Test peptide solubility using a small amount of the product, rather than the entire sample (Consideration to take into account when defining the quantity and the number of aliquots to order.Even if the solubility of SB-PEPTIDE peptides is tested, the guidelines below may be helpful in preparing peptide solutions.Īsk now for your peptide solubility calculator by submitting a request through the form on the right side of the page. Finding the ideal solvent to dissolve your peptide is often a challenge. Improper peptide solubilization results in inaccurate peptide concentration, which can introduce experimental errors into data or lead to experimental failure. The solubilization is a critical step for a successful peptide assay. SB-PEPTIDE’s custom synthesized peptides are delivered as lyophilized powder in 2 mL microtubes, that needs to be reconstituted.
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